Extraction method and pharmacological effects of magnolol

1. Extraction method

(1) Production process of Magnolia officinalis extract (liquid extract)

Take Magnolia officinalis and crush it and infiltrate it with ethanol for 12 hours, place it in a percolation cylinder, and percolate with about 12 times the amount of ethanol, collect the percolate, and recover the ethanol under reduced pressure until it is exhausted to obtain a liquid extract. The yield is about 9%, the solid content is 85.0%, the content of magnolol is more than 11.0%, and the content of honokiol is more than 5.0%.

(2) Extraction and separation of magnolol and honokiol Take the crude magnolia bark powder, add 1/5 amount (W/W) quicklime powder, mix well, and percolate with 15-20 times the amount of distilled water , Add salt to the percolate Chemicalbook to adjust the pH to 2～3, and let it stand. Collect the precipitated precipitate, wash it with distilled water until the pH of the precipitate is 6-7, add alumina (1:10) after drying and mix well, put it in an extractor, and extract with cycloethane. The cycloethane extract was concentrated and then left to cool to precipitate white crystals, which were filtered to obtain crystals and mother liquor. The crystallization is recrystallized with cyclohexane, which is honokiol. After the mother liquor is concentrated, crystals are precipitated, and cyclohexane is recrystallized to obtain colorless flake crystals, namely magnolol crystals. The yield of magnolol was 85%, and the yield of honokiol was 74%.

2. Pharmacological effects

(1) The role of anti-pathogenic microorganisms. Magnolia decoction against Staphylococcus, Streptococcus hemolyticus, Pneumococcus, Bacillus pertussis and other Gram-positive bacteria, as well as Bacillus anthracis, Shigella, Bacillus typhi, Bacillus paratyphi, Vibrio cholerae Bacteria, Escherichia coli, Proteus, Bacillus subtilis, Bacillus diphtheria and other Gram-negative bacteria have inhibitory effects, and the inhibitory effect on Staphylococcus is the strongest. At 15% concentration, it has inhibitory effect on dermatophytes such as Bacillus microsporum, Trichophyton concentric, Trichophyton rubrum, Trichophyton mellifera and other dermatophytes. Houpu decoction has certain effects on improving the substantial pathological damage of experimental viral hepatitis in mice. Magnolol has significant antibacterial activity against Gram-positive bacteria and acid-resistant bacteria; Magnolol has a significant anti-caries effect, with a minimum inhibitory concentration of 6.3μg/ml, and its antibacterial activity is stronger than typical antibacterial alkaloids. Berberine (MIC: 50μg/ml). The saturated aqueous solution of magnoline and Magnolia officinalis volatile oil has a certain inhibitory effect on Staphylococcus aureus, Sarcina and Bacillus subtilis.

(2) Effects on the cardiovascular system Magnolol and honokiol inhibit the formation of thromboxane B2 under various conditions, and the increase in intracellular Ca2+ caused by arachidonic acid or collagen is also inhibited by both. Honokiol can inhibit the activity of CaM-stimulated cyclic nucleotide phosphodiesterase. Honokiol can bind to CaM in the presence of Ca2+, thereby antagonizing its activation of phosphodiesterase. In addition, honokiol has a stimulating effect on the basic activity of CaM-dependent phosphodiesterase.

(3) Anti-tumor effect Magnolol and its hydroxymethyl derivatives have a significant inhibitory effect on the second-stage skin tumors in mice. The three extracting components of magnolia lignan, magnolol, honokiol and monoterpene magnolol are Epstein induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) -An antagonist of Barr virus early antigen (EBv-EA) activation. The methanol extract of Magnolia officinalis and magnolol have a significant inhibitory effect on mouse skin tumors caused by the second phase carcinogenic experiment in vivo.