The efficacy and contraindications of wolfberry polysaccharide

The effect of wolfberry in our kitchen and dinner table is very big. Whether it is stewed meat or making tea, we have to put a few wolfberries to improve the taste and use it as medicine. It can be used for health care. With economy, modern medicine and food With the development of science, people pay more and more attention to the role of food nutrition and health care. As one of my country's clearly defined foods with the same medicine and food, wolfberry has a health care function that cannot be underestimated.

1. Extraction and purification of Lycium barbarum polysaccharides

The extraction and purification of Lycium barbarum polysaccharides is generally divided into two steps. One is the extraction of Lycium barbarum crude polysaccharides, and the other is the fractionation and purification of Lycium barbarum polysaccharides. The extraction process of crude polysaccharide is as follows: Weigh the dried and crushed wolfberry fruit, use petroleum ether: acetone (1:1) to reflux and filter out the solvent, and the residue is air-dried to remove monosaccharides and oligosaccharides with 80% ethanol. The polysaccharide is extracted from the residue of de-oligosaccharide by water at 90~100℃, the extract is concentrated under reduced pressure and then ethanol is used to precipitate the polysaccharide, and then the precipitate is washed with absolute ethanol and acetone, and then dried in vacuum to obtain crude wolfberry polysaccharide. Fractional purification of crude polysaccharides--pass Lycium barbarum crude polysaccharides through a DEAE cellulose column, gradient elution with different concentrations of NaCl, and the eluates with different salt concentrations are concentrated under reduced pressure and then desalted by dialysis and freeze-dried to obtain different Fraction of Lycium barbarum glycoprotein.

2. The effect of Lycium barbarum polysaccharide

(1) Anti-oxidation and anti-aging

The accumulation of oxidative stress will promote the process of aging. The anti-aging experiments of Lycium barbarum polysaccharides are mostly used for free radicals as carriers of toxins. It has been reported that Lycium barbarum polysaccharides exhibit antioxidant capacity in various antioxidant systems in vitro.

(2) Immune regulation

Lycium barbarum polysaccharides affect the function of immune regulation in many ways. By further separating and purifying crude polysaccharides by ion exchange chromatography, a proteoglycan complex, Lycium barbarum polysaccharide 3p, can be obtained, which has an immunostimulatory effect.

(3) Anti-tumor effect.

Lycium barbarum polysaccharide 3p can inhibit the growth of transplanted S180 sarcoma, increase the phagocytic ability of macrophages, the proliferation of spleen macrophages and the secretion of antibodies of spleen cells, impair the viability of T macrophages, the expression of IL2mRNA and reduce lipids Peroxidation.

(4) Anti-tumor

Lycium barbarum polysaccharide can inhibit the growth of some tumors, but there are different opinions on its mechanism. Some people believe that Lycium barbarum polysaccharide can activate the body's immune system to achieve anti-tumor goals, but its anti-tumor effect is still lacking reliable evidence. 

(5) Regulate blood lipids and lower blood sugar

Lycium barbarum polysaccharides can reduce blood sugar and serum MDA and nitric oxide content in non-insulin-dependent diabetic rats, and can significantly increase the serum SOD content and reduce the DNA damage of peripheral lymphocytes. Lycium barbarum polysaccharides can reduce the blood glucose and blood lipid levels of alloxan-induced diabetic rabbits, and lower the blood lipid and blood sugar levels.

(6) Anti-radiation

Lycium barbarum polysaccharides can promote the recovery of peripheral blood in myelosuppressed mice caused by X-ray and carboplatin chemotherapy, and can stimulate human peripheral blood mononuclear cells to produce recombinant granulocyte colony stimulating factor; radiation can cause membrane damage to the mitochondria of mouse liver cells. Lycium barbarum polysaccharide can reduce this damage, improve the loss of mitochondrial sulfhydryl protein in liver cells caused by radiation, and the inactivation of SOD, catalase and GSHPx, and its anti-radiation function is more obvious than tocopherol.

(7) Neuroprotection

The research of the University of Hong Kong found that the extract of wolfberry can play a neuroprotective effect by resisting the stress level of the endoplasmic reticulum of nerve cells, and the polysaccharide of wolfberry plays a protective role against the neurotoxicity caused by amyloid peptide. Amyloid peptide is one of the factors that promote neuron loss in the occurrence of Alzheimer's disease. Lycium barbarum polysaccharide can reduce the phosphate of c-junk amino terminal kinase (JNK) by reducing the secretion of lactate dehydrogenase and inhibiting the activity of amyloid peptide protease. It can prevent the apoptosis of nerve cells and the phosphorylation of RNA-dependent protein kinase, which can play a neuroprotective role, and may play a role in the occurrence of Alzheimer's disease.

(8) Anti-cancer effect

Observation of the biological effects of Lycium barbarum on cancer cells by cell culture in vitro has proved that Lycium barbarum has a significant inhibitory effect on human gastric adenocarcinoma KATO-I cells and human cervical cancer Hela cells. Lycium barbarum polysaccharides has been used to treat 20 cases of primary liver cancer, which can improve the symptoms and the condition of low immune function and prolong survival.

3. It should be noted that those with the following symptoms should use wolfberry with caution

(1) If the spleen and stomach are weak and cold, eating wolfberry will cause diarrhea.

(2) If you are having a cold, fever, body inflammation, or patients with symptoms of spleen and stomach deficiency and diarrhea, please eat less or refrain from eating.